Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a lucia software

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F01%3A00005622" target="_blank" >RIV/00216224:14110/01:00005622 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a lucia software

Original language description

The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM), produced by Schwann cells and fibroblasts under control of the axons, is important extrinsic factor of the nerve regeneration. A different content of the endoneurial ECM can be expected in the dorsal and ventral spinal roots in relation to their axon type differences, but no information is available to the present time. In the present paper we demonstrated a comparison of immunofluorescenceintensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of the rat dorsal and ventral spinal roots. For this purpose the cryostat sections through the dorsal and ventral roots were cut and incubatedsimultaneously. We tested optimal incubation conditions with the primary and secondary antibodies to distinguish differences in immunostaining intensity of the endoneurial ECM for investigated molecules. The best results of distinct stai

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

<a href="/en/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Cellular and extracellular environment for the axon regeneration following injury of the spinal roots and peripheral nerves</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The Histochemical Journal

ISSN

0018-2214

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

8

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

475

Pages from-to

475

UT code for WoS article

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EID of the result in the Scopus database

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