Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F03%3A00012310" target="_blank" >RIV/00216224:14110/03:00012310 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

Original language description

DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum(Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicati

Czech name

Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků

Czech description

Anotace česky: Byl vyšetřen transkriptom Treponema pallidum během experimentální infekce králíků pomocí DNA microarray čipů obsahujících všech 1039 anotovaných ORF. Geny Treponema pallidum byly rozděleny do skupin podle úrovně transkripce.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/NI7351" target="_blank" >NI7351: Comparative genomics of pathogenic spirochetes of the genus Treponema: the route towards sequence-specific diagnostics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CHEMICKÉ LISTY

ISSN

0009-2770

e-ISSN

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Volume of the periodical

2003

Issue of the periodical within the volume

97

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

311

UT code for WoS article

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EID of the result in the Scopus database

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