Antiproliferative activities of selected benzo[c]phenanthridine alkaloids.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F05%3A00013263" target="_blank" >RIV/00216224:14110/05:00013263 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Antiproliferative activities of selected benzo[c]phenanthridine alkaloids.

Original language description

Quaternary benzo[c]phenanthridine alkaloids (QBA) fall into a group of isochinoline alkaloids that have phenylalanin as their precursor. They are isolated from many plant species for instance from Papaveraceae, Fumariaceae and Rutaceae families. Sanguinarin and chelerytrin are the two best known QBA and their biological activities have been widely studied (1,2,3). In addition to them about 10 other QBA differing only by substitution on the aromatic rings have been isolated as a minor components in several species. The informations about biological effects of this minor QBA are rare. In this study the biological effect of sanguinarine (SA), chelerythrine (CHE) and their derinates sanguirubine (SR), chelirubine (CHR) and macarpine (MA) were tested. One normal (skin fibroblasts) and 3 tumour cell lines (HeLa; A 431; HL-60) were used as a model object. Cytotoxicity (IC50) and apoptosis on living cells were studied. After 72 hours, MTT assay was performed to evaluate the in vitro cytotoxici

Czech name

Antiproliferativn inky vybranch benzo[c]phenanthridinovch alkaloid

Czech description

Kvartern benzo[c]phenanthridine alkaloids (QBA) pat do skupiny isochinolinovch alkaloid, jejich prekursorem je phenylalanin. Vyskytuj se v rostlinch eled Papaveraceae, Fumariaceae a Rutaceae. Nejznmj a nejlpe prostudovan jsou Sanguinarin and chelerytrin,informace o biologickch aktivitch minoritnch derivt jsou vzcn. Tato studie se zabv srovnnm antiproliferan aktivity sanguinarinu (SA), chelerythrinu (CHE) a minoritnch derivt sanguirubinu (SR), chelirubinu (CHR) a macarpinu (MA) na linii normlnch lidskchkonch fibroblast a 3 ndorov bunn linie (HeLa; A 431; HL-60). Antiproliferan aktivita byla stanovena po 72 hodinov kultivaci pomoc MTT assae, schopnost indukovat apoptzu byla studovna pomoc kombinace Anexinu V a propidium jodidu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

149

Issue of the periodical within the volume

Suppl.1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

2

Pages from-to

57-58

UT code for WoS article

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EID of the result in the Scopus database

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