Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00016449" target="_blank" >RIV/00216224:14110/06:00016449 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/06:00040611

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.

Original language description

We suggest a method for the reproducible and efficient capillary isoelectric focusing of proteins and microorganisms in the pH gradient 3-10. The method involves the segmental injection of the simple ampholytes, the solution of the selected electrolytes,and the sample mixture of bioanalytes and carrier ampholytes to the fused silica capillaries dynamically modified by poly(ethylene glycol), PEG 4000, which is added to the catholyte, the anolyte and injected solutions. In order to receive the reproducible results, the capillaries were rinsed by the mixture of acetone/ethanol between analyses. For the tracing of the pH gradients the low-molecular-mass pI markers were used. The simple proteins and the mixed cultures of microorganisms, Saccharomyces cerevisiae CCM 8191, Escherichia coli CCM 3954, Candida albicans CCM 8180, Candida parapsilosis, Candida krusei, Staphylococcus aureus, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224, Staphylococcus epidermidis CCM 4418 and

Czech name

CIEF proteinů a mikroorganizmů v dynamicky modifikované křemičité kapiláře s UV detekcí.

Czech description

Navržená CIEF metoda umožňuje citlivě a reprodukovatelně v gradientu pH 3-10 detekovat a identifikovat proteiny i mikroorganizmy a to jak z čistých kultur, tak ze směsí.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FN - Epidemiology, infection diseases and clinical immunology

OECD FORD branch

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Project

<a href="/en/project/IAA4031302" target="_blank" >IAA4031302: Using of the fast electrophoretic separation with the high sensitive fluorometric detection for the identification of micro-organisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Volume of the periodical

841

Issue of the periodical within the volume

1-2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

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UT code for WoS article

000240393200017

EID of the result in the Scopus database

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