Significance of microRNA-21 in colorectal cancer pathogenesis.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00028239" target="_blank" >RIV/00216224:14110/08:00028239 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Significance of microRNA-21 in colorectal cancer pathogenesis.

Original language description

MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. Expression levels of miR-21 were

Czech name

Significance of microRNA-21 in colorectal cancer pathogenesis.

Czech description

MicroRNAs (miRNAs) are endogenously expressed short non-coding RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in tumors of 29 colorectal cancer patients. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. Expression levels of miR-21 were

Type

D - Article in proceedings

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NR9076" target="_blank" >NR9076: Gene expression profiling-based prediction of treatment response for patient with locally advanced rectal cancer treated by chemotherapy and radiotherapy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

FEBS Journal

ISBN

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ISSN

1742-464X

e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

FEBS

Place of publication

Oxford, UK

Event location

Athens

Event date

Jan 1, 2008

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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