Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00028238" target="_blank" >RIV/00216224:14110/08:00028238 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Original language description

Although the number of verified human microRNAs (miRNAs) is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous data suggest altered regulation of microRNA-21 (miR-21) expression in CRC. In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in 60 colorectal tumors and40 paired adjacent non-tumor tissues and correlated them to selected clinicopathologic features and survival parameters. We used expression of U6 small nuclear RNA (RNU6B) for data normalization and standard ddCt method for relative quantification of miRNA expression. Levels of miR-21 were significantly higher in tumors comparing to normal mucosa (p &lt; 0,0001, Wilcoxon matched-pairs test). High expression levels of miR-21 in tumors (based on high tertile) were associated also with a p

Czech name

Knockdown of oncogenic microRNA-21 displays cytotoxicity in p53 wild-type colon cancer cells

Czech description

Although the number of verified human microRNAs (miRNAs) is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous data suggest altered regulation of microRNA-21 (miR-21) expression in CRC. In our study, we examined by Real-Time PCR expression levels of microRNA-21 (miR-21) in 60 colorectal tumors and40 paired adjacent non-tumor tissues and correlated them to selected clinicopathologic features and survival parameters. We used expression of U6 small nuclear RNA (RNU6B) for data normalization and standard ddCt method for relative quantification of miRNA expression. Levels of miR-21 were significantly higher in tumors comparing to normal mucosa (p &lt; 0,0001, Wilcoxon matched-pairs test). High expression levels of miR-21 in tumors (based on high tertile) were associated also with a p

Type

D - Article in proceedings

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NR9076" target="_blank" >NR9076: Gene expression profiling-based prediction of treatment response for patient with locally advanced rectal cancer treated by chemotherapy and radiotherapy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

European Journal of Cancer Supplements

ISBN

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ISSN

1359-6349

e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

Elsevier Ltd.

Place of publication

Oxford, UK

Event location

Lyon, France

Event date

Aug 5, 2008

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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