Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00029527" target="_blank" >RIV/00216224:14110/09:00029527 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method
Original language description
The influence of ischemia and reperfusion duration on left ventricular depolarization is studied from electrograms (ECG) and monophasic action potentials (MAPs). Three orthogonal ECG signals and MAPs are recorded simultaneously during ischemia in isolated rabbit hearts perfused according to Langendorff. MAPs are recorded by optical method via fluorescence measurement from the heart surface at appointed place. The principle of optical measurement of MAP is an application of voltage-sensitive dye (VSD), di-4-ANEPPS in our experimental set-up, into the heart tissue. Optical signal therefore depends on cardiac action potential. The ratiometric fluorescence method, based on emitted light detection by a segment narrow-band photodiode at two different wavelengths, is used. MAPs are recorded with high signal-to-noise ratio and at 2kHz sampling frequency.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů