Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00049626" target="_blank" >RIV/00216224:14110/09:00049626 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method

Original language description

The influence of ischemia and reperfusion duration on left ventricular depolarization was studied from electrograms (ECG) and monophasic action potentials (MAPs). Three orthogonal ECG signals and MAPs were recorded simultaneously during ischemia in isolated rabbit hearts perfused according to Langendorff. MAPs were recorded by optical method via fluorescence measurement from the heart surface at appointed place. The principle of optical measurement of MAP is an application of voltage-sensitive dye (VSD), di-4-ANEPPS in our experimental set-up, into the heart tissue. Optical signal therefore depends on cardiac action potential. The ratiometric fluorescence method, based on emitted light detection by a segment narrow-band photodiode at two different wavelengths, was used. MAPs were recorded with high signal-to-noise ratio and at 2kHz sampling frequency. The speed of depolarization propagation in ventricular tissue was derived from activation time (AT).

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

—

Project

<a href="/en/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Computers in Cardiology

ISSN

0276-6574

e-ISSN

—

Volume of the periodical

36

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

525-528

UT code for WoS article

—

EID of the result in the Scopus database

—