Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00040436" target="_blank" >RIV/00216224:14110/10:00040436 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Functional analysis of the common haplotype in the Receptor for Advanced Glycation End-products gene previously identified as a susceptibility factor for diabetic nephropathy

Original language description

The Receptor for Advanced Glycation End-products (RAGE) belongs to the family of pattern-recognition receptors and is significantly involved in the molecular mechanisms mediating pro-inflammatory action of hyperglycemia in diabetes. The aim of the current study was to elucidate the possible functional impact of the genetic variability in the AGER gene constituting previously identified risk haplotype for diabetic nephropathy (-429C/-374T/2184G) by testing the haplotype-specific effect on the AGER gene transcriptional activity in vitro. Promotor and intron 8 constructs carrying respective substitutions were amplified and cloned into pGL3-Basic reporter vector and subsequently used for transfection of HEK293 cells. Following 48hrs incubation in either normo- (5 mM/L) or hyperglycemic (25 mM/L) culture medium luciferase activity was measured to assess transcriptional efficiency.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

<a href="/en/project/KJB501620601" target="_blank" >KJB501620601: Functional analysis of the RAGE gene susceptibility haplotype and its role in the hyperglycemia-driven pathology</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental and Clinical Endocrinology & Diabetes

ISSN

0947-7349

e-ISSN

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Volume of the periodical

118

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

3

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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