Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00040816" target="_blank" >RIV/00216224:14110/10:00040816 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.

Original language description

The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL1 and Mexico A), T. p. pertenue strains (Samoa D, CDC2 and Gauthier), and the FribourgBlanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate genome sequence identity investigated based on this whole genome fingerprinting (WGF) analysis ranged from 99.57 to 99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains and could be used for the molecular detection.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PloS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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