Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00049878" target="_blank" >RIV/00216224:14110/11:00049878 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/11:#0000764
Result on the web
<a href="http://dx.doi.org/10.1016/j.dnarep.2011.03.003" target="_blank" >http://dx.doi.org/10.1016/j.dnarep.2011.03.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dnarep.2011.03.003" target="_blank" >10.1016/j.dnarep.2011.03.003</a>
Alternative languages
Result language
angličtina
Original language name
Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities
Original language description
The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. While some of the aspects of the initial steps of HR are understood, the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. Therefore, we have reconstituted D-loop-based in vitro recombinationassociated DNA repair synthesis assay and tested the efficacy of polymerases Pol and Pol to extend invaded primer, and the ability of three helicases (Mph1, Srs2 and Sgs1) to displace this extended primer. Both Pol and Pol extended up to 50% of the D-loop substrate, but differed in product length and dependency on proliferating cell nuclear antigen (PCNA). Mph1, but not Srs2 or Sgs1, displaced the extended primer very efficiently, supporting putative role ofMph1in promoting the synthesis-dependent strand-annealing pathway.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
DNA Repair
ISSN
1568-7864
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
567-576
UT code for WoS article
000292440800002
EID of the result in the Scopus database
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