Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00066407" target="_blank" >RIV/00216224:14110/13:00066407 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/13:00060671
Result on the web
<a href="http://dx.doi.org/10.1016/j.dnarep.2013.05.001" target="_blank" >http://dx.doi.org/10.1016/j.dnarep.2013.05.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dnarep.2013.05.001" target="_blank" >10.1016/j.dnarep.2013.05.001</a>
Alternative languages
Result language
angličtina
Original language name
Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
Original language description
Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
DNA Repair
ISSN
1568-7864
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
9
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
691-698
UT code for WoS article
000323468400001
EID of the result in the Scopus database
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