Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00066406" target="_blank" >RIV/00216224:14110/13:00066406 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/13:00060678
Result on the web
<a href="http://dx.doi.org/10.1038/emboj.2013.9" target="_blank" >http://dx.doi.org/10.1038/emboj.2013.9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/emboj.2013.9" target="_blank" >10.1038/emboj.2013.9</a>
Alternative languages
Result language
angličtina
Original language name
Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis
Original language description
Completion of DNA replication needs to be ensured even when challenged with fork progression problems or DNA damage. PCNA and its modifications constitute a molecular switch to control distinct repair pathways. In yeast, SUMOylated PCNA (S-PCNA) recruitsSrs2 to sites of replication where Srs2 can disrupt Rad51 filaments and prevent homologous recombination (HR). We report here an unexpected additional mechanism by which S-PCNA and Srs2 block the synthesis-dependent extension of a recombination intermediate, thus limiting its potentially hazardous resolution in association with a cross-over. This new Srs2 activity requires the SUMO interaction motif at its C-terminus, but neither its translocase activity nor its interaction with Rad51. Srs2 binding toS-PCNA dissociates Pol delta and Pol eta from the repair synthesis machinery, thus revealing a novel regulatory mechanism controlling spontaneous genome rearrangements.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO JOURNAL
ISSN
0261-4189
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
742-755
UT code for WoS article
000316468000014
EID of the result in the Scopus database
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