Unloading of homologous recombination factors is required for restoring double‐stranded DNA at damage repair loci

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00066065" target="_blank" >RIV/00159816:_____/17:00066065 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00094600

Result on the web

<a href="http://dx.doi.org/10.15252/embj.201694628" target="_blank" >http://dx.doi.org/10.15252/embj.201694628</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15252/embj.201694628" target="_blank" >10.15252/embj.201694628</a>

Result language

angličtina

Original language name

Unloading of homologous recombination factors is required for restoring double‐stranded DNA at damage repair loci

Original language description

Cells use homology-dependent DNA repair to mend chromosome breaks and restore broken replication forks, thereby ensuring genome stability and cell survival. DNA break repair via homology-based mechanisms involves nuclease-dependent DNA end resection, which generates long tracts of single-stranded DNA required for checkpoint activation and loading of homologous recombination proteins Rad52/51/55/57. While recruitment of the homologous recombination machinery is well characterized, it is not known how its presence at repair loci is coordinated with downstream re-synthesis of resected DNA. We show that Rad51 inhibits recruitment of proliferating cell nuclear antigen (PCNA), the platform for assembly of the DNA replication machinery, and that unloading of Rad51 by Srs2 helicase is required for efficient PCNA loading and restoration of resected DNA. As a result, srs2Δ mutants are deficient in DNA repair correlating with extensive DNA processing, but this defect in srs2Δ mutants can be suppressed by inactivation of the resection nuclease Exo1. We propose a model in which during re-synthesis of resected DNA, the replication machinery must catch up with the preceding processing nucleases, in order to close the single-stranded gap and terminate further resection.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Embo Journal

ISSN

0261-4189

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

19

Pages from-to

213-231

UT code for WoS article

000393317800008

EID of the result in the Scopus database

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