Human CtIP promotes DNA end resection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004915" target="_blank" >RIV/61989592:15310/07:00004915 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/07:00010288 RIV/61989592:15110/07:00009738
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Human CtIP promotes DNA end resection
Original language description
In the S and G2 phases of the cell cycle, DNA double-strand breaks (DSBs) are processed into single-stranded DNA, triggering ATR-dependent checkpoint signalling and DSB repair by homologous recombination. Previous work has implicated the MRE11 complex insuch DSB-processing events. Here, we show that the human CtIP (RBBP8) protein confers resistance to DSB-inducing agents and is recruited to DSBs exclusively in the S and G2 cell-cycle phases. Moreover, we reveal that CtIP is required for DSB resection,and thereby for recruitment of replication protein A (RPA) and the protein kinase ATR to DSBs, and for the ensuing ATR activation. Furthermore, we establish that CtIP physically and functionally interacts with the MRE11 complex, and that both CtIP and MRE11 are required for efficient homologous recombination. Finally, we reveal that CtIP has sequence homology with Sae2, which is involved in MRE11-dependent DSB processing in yeast. These findings establish evolutionarily conserved roles f
Czech name
Lidský CtIP způsobuje koncovou resekci DNA
Czech description
Zjištili jsme evolučně konzervativní roli proteinů podobných CtIP při kontrole DSB resekce, signalizace kontrolního bodu a homologních rekombinací.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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