CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33139411" target="_blank" >RIV/61989592:15110/12:33139411 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/embor.2012.58" target="_blank" >http://dx.doi.org/10.1038/embor.2012.58</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/embor.2012.58" target="_blank" >10.1038/embor.2012.58</a>
Alternative languages
Result language
angličtina
Original language name
CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination
Original language description
The conserved MRE11-RAD50-NBS1 (MRN) complex is an important sensor of DNA double-strand breaks (DSBs) and facilitates DNA repair by homologous recombination (HR) and end joining. Here, we identify NBS1 as a target of cyclin-dependent kinase (CDK) phosphorylation. We show that NBS1 serine 432 phosphorylation occurs in the S, G2 and M phases of the cell cycle and requires CDK activity. This modification stimulates MRN-dependent conversion of DSBS into structures that are substrates for repair by HR. Impairment of NBS1 phosphorylation not only negatively affects DSB repair by HR, but also prevents resumption of DNA replication after replication-fork stalling. Thus, CDK-mediated NBS1 phosphorylation defines a molecular switch that controls the choice of repair mode for DSBs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO REPORTS
ISSN
1469-221X
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
561-568
UT code for WoS article
000305069400019
EID of the result in the Scopus database
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