LEDGF (p75) promotes DNA-end resection and homologous recombination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33139402" target="_blank" >RIV/61989592:15110/12:33139402 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/nsmb.2314" target="_blank" >http://dx.doi.org/10.1038/nsmb.2314</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nsmb.2314" target="_blank" >10.1038/nsmb.2314</a>

Result language

angličtina

Original language name

LEDGF (p75) promotes DNA-end resection and homologous recombination

Original language description

Lens epithelium derived growth factor p75 splice variant (LEDGF) is a chromatin-binding protein known for its antiapoptotic activity and ability to direct human immunodeficiency virus into active transcription units. Here we show that LEDGF promotes therepair of DNA double-strand breaks (DSBs) by the homologous recombination repair pathway. Depletion of LEDGF impairs the recruitment of C-terminal binding protein interacting protein (CtIP) to DNA DSBs and the subsequent CtIP-dependent DNA-end resection.LEDGF is constitutively associated with chromatin through its Pro-Trp-Trp-Pro (PWWP) domain that binds preferentially to epigenetic methyl-lysine histone markers characteristic of active transcription units. LEDGF binds CtIP in a DNA damage dependent manner, thereby enhancing its tethering to the active chromatin and facilitating its access to DNA DSBs. These data highlight the role of PWWP-domain proteins in DNA repair and provide a molecular explanation for the antiapoptotic and cance

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

NATURE STRUCTURAL & MOLECULAR BIOLOGY

ISSN

1545-9993

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

803-810

UT code for WoS article

000307153600012

EID of the result in the Scopus database

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