Multivalency of nucleosome recognition by LEDGF

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00575033" target="_blank" >RIV/61388963:_____/23:00575033 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/23:00575033 RIV/00216224:14740/23:00133766 RIV/00216208:11310/23:10471752

Result on the web

<a href="https://doi.org/10.1093/nar/gkad674" target="_blank" >https://doi.org/10.1093/nar/gkad674</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkad674" target="_blank" >10.1093/nar/gkad674</a>

Result language

angličtina

Original language name

Multivalency of nucleosome recognition by LEDGF

Original language description

Eukaryotic transcription is dependent on specific histone modifications. Their recognition by chromatin readers triggers complex processes relying on the coordinated association of transcription regulatory factors. Although various modification states of a particular histone residue often lead to differential outcomes, it is not entirely clear how they are discriminated. Moreover, the contribution of intrinsically disordered regions outside of the specialized reader domains to nucleosome binding remains unexplored. Here, we report the structures of a PWWP domain from transcriptional coactivator LEDGF in complex with the H3K36 di- and trimethylated nucleosome, indicating that both methylation marks are recognized by PWWP in a highly conserved manner. We identify a unique secondary interaction site for the PWWP domain at the interface between the acidic patch and nucleosomal DNA that might contribute to an H3K36-methylation independent role of LEDGF. We reveal DNA interacting motifs in the intrinsically disordered region of LEDGF that discriminate between the intra- or extranucleosomal DNA but remain dynamic in the context of dinucleosomes. The interplay between the LEDGF H3K36-methylation reader and protein binding module mediated by multivalent interactions of the intrinsically disordered linker with chromatin might help direct the elongation machinery to the vicinity of RNA polymerase II, thereby facilitating productive elongation.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Volume of the periodical

51

Issue of the periodical within the volume

18

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

10011-10025

UT code for WoS article

001163598900001

EID of the result in the Scopus database

2-s2.0-85175202376