Dual roles of the SUMO-interacting motif in the regulation of Srs2 sumoylation.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057621" target="_blank" >RIV/00216224:14110/12:00057621 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/12:#0000940

Result on the web

<a href="http://nar.oxfordjournals.org/content/40/16/7831.long" target="_blank" >http://nar.oxfordjournals.org/content/40/16/7831.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks484" target="_blank" >10.1093/nar/gks484</a>

Result language

angličtina

Original language name

Dual roles of the SUMO-interacting motif in the regulation of Srs2 sumoylation.

Original language description

The Srs2 DNA helicase of Saccharomyces cerevisiae affects recombination in multiple ways. Srs2 not only inhibits recombination at stalled replication forks but also promotes the synthesis-dependent strand annealing (SDSA) pathway of recombination. Both functions of Srs2 are regulated by sumoylation-sumoylated PCNA recruits Srs2 to the replication fork to disfavor recombination, and sumoylation of Srs2 can be inhibitory to SDSA in certain backgrounds. To understand Srs2 function, we characterize the mechanism of its sumoylation in vitro and in vivo. Our data show that Srs2 is sumoylated at three lysines, and its sumoylation is facilitated by the Siz SUMO ligases. We also show that Srs2 binds to SUMO via a C-terminal SUMO-interacting motif (SIM). The SIMregion is required for Srs2 sumoylation, likely by binding to SUMO-charged Ubc9. Srs2's SIM also cooperates with an adjacent PCNA-specific interaction site in binding to sumoylated PCNA to ensure the specificity of the interaction.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

16

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

7831-7843

UT code for WoS article

000308959800028

EID of the result in the Scopus database

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