Pro-recombination role of Srs2 requires SUMO but is independent of PCNA interaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00064071" target="_blank" >RIV/00159816:_____/16:00064071 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M115.685891" target="_blank" >http://dx.doi.org/10.1074/jbc.M115.685891</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M115.685891" target="_blank" >10.1074/jbc.M115.685891</a>

Result language
angličtina
Original language name
Pro-recombination role of Srs2 requires SUMO but is independent of PCNA interaction
Original language description
Srs2 plays many roles in DNA repair, the proper regulation and coordination of which is essential. Post-translational modification by Small Ubiquitin-like Modifier (SUMO) is one such possible mechanism. Here, we investigate SUMO's role in Srs2 regulation and show that the SUMO-interacting motif (SIM) of Srs2 is important for the interaction with several recombination factors. Lack of SIM, but not PCNA-interacting motif (PIM), leads to increased cell death under circumstances requiring homologous recombination for DNA repair. Simultaneous mutation of SIM in a srs2dPIM strain leads to a decrease in recombination, indicating a pro-recombination role of SUMO. Thus SIM has an ambivalent function in Srs2 regulation, it not only mediates interaction with SUMO-PCNA to promote the anti recombination function but it also plays a PCNA-independent pro-recombination role, probably by stimulating the formation of recombination complexes. The fact that deletion of PIM suppresses the phenotypes of Srs2 lacking SIM suggests that proper balance between the anti-recombination PCNA-bound and pro-recombination pools of Srs2 is crucial. Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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