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Pro-recombination role of Srs2 requires SUMO but is independent of PCNA interaction

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00064071" target="_blank" >RIV/00159816:_____/16:00064071 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1074/jbc.M115.685891" target="_blank" >http://dx.doi.org/10.1074/jbc.M115.685891</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1074/jbc.M115.685891" target="_blank" >10.1074/jbc.M115.685891</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Pro-recombination role of Srs2 requires SUMO but is independent of PCNA interaction

  • Original language description

    Srs2 plays many roles in DNA repair, the proper regulation and coordination of which is essential. Post-translational modification by Small Ubiquitin-like Modifier (SUMO) is one such possible mechanism. Here, we investigate SUMO's role in Srs2 regulation and show that the SUMO-interacting motif (SIM) of Srs2 is important for the interaction with several recombination factors. Lack of SIM, but not PCNA-interacting motif (PIM), leads to increased cell death under circumstances requiring homologous recombination for DNA repair. Simultaneous mutation of SIM in a srs2dPIM strain leads to a decrease in recombination, indicating a pro-recombination role of SUMO. Thus SIM has an ambivalent function in Srs2 regulation, it not only mediates interaction with SUMO-PCNA to promote the anti recombination function but it also plays a PCNA-independent pro-recombination role, probably by stimulating the formation of recombination complexes. The fact that deletion of PIM suppresses the phenotypes of Srs2 lacking SIM suggests that proper balance between the anti-recombination PCNA-bound and pro-recombination pools of Srs2 is crucial. Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biological Chemistry

  • ISSN

    0021-9258

  • e-ISSN

  • Volume of the periodical

    291

  • Issue of the periodical within the volume

    14

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    7594-7607

  • UT code for WoS article

    000383447600032

  • EID of the result in the Scopus database