In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00052566" target="_blank" >RIV/00216224:14110/11:00052566 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/11:00370061 RIV/68378050:_____/11:00370061
Result on the web
<a href="http://dx.doi.org/10.1007/s10571-011-9669-2" target="_blank" >http://dx.doi.org/10.1007/s10571-011-9669-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10571-011-9669-2" target="_blank" >10.1007/s10571-011-9669-2</a>
Alternative languages
Result language
angličtina
Original language name
In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
Original language description
Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced underdifferentiation conditions in quickly formed embryoid bodies (qEBs) after 10?12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocolsusing normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA204%2F08%2F1618" target="_blank" >GA204/08/1618: Molecular basis of canonical Wnt signalling during eye and brain development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular and Molecular Neurobiology
ISSN
0272-4340
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
715-727
UT code for WoS article
000290914300008
EID of the result in the Scopus database
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