LPS structure influences protein secretion in Salmonella enterica

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00052915" target="_blank" >RIV/00216224:14110/11:00052915 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/11:#0000747 RIV/00216224:14310/11:00081770

Result on the web

<a href="http://dx.doi.org/10.1016/j.vetmic.2011.04.018" target="_blank" >http://dx.doi.org/10.1016/j.vetmic.2011.04.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetmic.2011.04.018" target="_blank" >10.1016/j.vetmic.2011.04.018</a>

Result language

angličtina

Original language name

LPS structure influences protein secretion in Salmonella enterica

Original language description

In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Center for Advanced Microbiology & Immunology Research in Veterinary Medicine</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Volume of the periodical

152

Issue of the periodical within the volume

1

Country of publishing house

IE - IRELAND

Number of pages

7

Pages from-to

131-137

UT code for WoS article

000294095000015

EID of the result in the Scopus database

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