VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00057214" target="_blank" >RIV/00216224:14110/11:00057214 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
VISUALISATION OF A PROTEIN DISTRIBUTION BY USING THE FREEZE-ETCHING METHOD
Original language description
The freeze-etching is one of cryo-methods, which is used to visualize the membrane structure of the cells. In general, this method provides information about the protrusions of the cell membrane or the undulation of surface of the plasma membrane, but bythe processing of samples it is very common to achieve fracturing through the organelles inside the cells, i.e. mitochondria, endoplasmic reticulum or nuclear envelope with nuclear pores. As the membranes are usually fractured on their protoplasmic or exoplasmic face, it is possible to observe the trans-membrane or intra-membrane proteins without the need of specific labelling. The replica of membranes is 25 nm thick, and is formed by a thin layer of platinum and carbon.
Czech name
—
Czech description
—

Project
<a href="/en/project/GPP506%2F10%2FP372" target="_blank" >GPP506/10/P372: Comparative morphology and immunohistochemistry in the evaluation of phylogenetic affinities among early emerging apicomplexans</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)