Freeze-fracture technique and artefacts caused by processing conditions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00434127" target="_blank" >RIV/68081731:_____/14:00434127 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Freeze-fracture technique and artefacts caused by processing conditions
Original language description
Freeze-fracture technique is a method used to visualise membrane surfaces of cell organelles. This method is based on cryo-fixation that stabilizes samples. The sample is rapidly frozen in nitrogen, and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant preserving the fine structure of cells in their native stage. Although, cryoprotectants serve as a substitute for water and protect against ice crystal production, they could also affect the form of fracture through biological membranes. Figure 1 shows structures in a sample frozen in the presence of 25% glycerol. The temperature of the apparatus was not low enough during the process of fracturing and etching the sample. The structure of cells seems to be deformed due to melting glycerol. In contrast, figure 2 shows a replica with fine structure of frozen and proper good form of fracturing. The cells used for this study were human leukemic cells (HL-60). Another artefact is shown in figure 3A, co
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
18th International Microscopy Congres. Proceedings
ISBN
978-80-260-6720-7
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
Czechoslovak Microscopy Society
Place of publication
Praha
Event location
Praha
Event date
Sep 7, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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