Freezer-fracture technique and artefacts caused by processing conditions.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073934" target="_blank" >RIV/00216224:14310/14:00073934 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Freezer-fracture technique and artefacts caused by processing conditions.
Original language description
Freeze-fracture technique is method for visualisation membrane surfaces of cell organelles. This method used cryo-fixation for stabilization a sample. Sample is frozen in nitrogen and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant for observation fine and smooth cellular structures. Although, cryoprotectants serve for the substitution water and elimination ice crystals production, they could affect a form of fracture through biological membranes and could affect interpretation of morphological structures.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EG - Zoology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP506%2F12%2F1258" target="_blank" >GAP506/12/1258: Host-parasite interactions in blood-feeding diplozoid monogeneans: Investigation of highly specialized adaptation for parasitism</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů