Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057446" target="_blank" >RIV/00216224:14110/12:00057446 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/12:00188138 RIV/00216305:26620/12:PU99119 RIV/62157124:16370/12:43871328

Result on the web

<a href="http://dx.doi.org/10.1039/c2ib00157h" target="_blank" >http://dx.doi.org/10.1039/c2ib00157h</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2ib00157h" target="_blank" >10.1039/c2ib00157h</a>

Result language

angličtina

Original language name

Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining

Original language description

The present paper is focused on zinc(II) treatment effects on prostatic cell lines PC-3 (tumour) and PNT1A (non-tumour). Oxidative status of cells was monitored by evaluation of expression of metallothionein (MT) isoforms 1A and 2A at the mRNA and protein level, glutathione (oxidised and reduced), and intracellular zinc(II) after exposition to zinc(II) treatment at concentrations of 0?150 mM using electrochemical methods, western blotting and fluorescent microscopy. A novel real-time impedance-based growth monitoring system was compared with widely used end-point MTT assay. Impedance-based IC50 for zinc(II) is 55.5 and 150.8 mM for PC-3 and PNT1A, respectively. MTTdetermined IC50 are &gt;1.3-fold higher. Impedance-based viability correlates with viablecount (r &gt; 0.92; p o 0.03), not with MTT. Two-fold lower intracellular zinc(II) in the tumour PC-3 cell line was found. After zinc(II) treatment &gt;2.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Integrative Biology

ISSN

1757-9694

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

672-684

UT code for WoS article

000304487300010

EID of the result in the Scopus database

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