Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057556" target="_blank" >RIV/00216224:14110/12:00057556 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00389633

Result on the web

<a href="http://dx.doi.org/10.1515/hsz-2011-0279" target="_blank" >http://dx.doi.org/10.1515/hsz-2011-0279</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/hsz-2011-0279" target="_blank" >10.1515/hsz-2011-0279</a>

Result language

angličtina

Original language name

Necroptosis modulated by autophagy is a predominant form of melanoma cell death induced by sanguilutine

Original language description

We show that the plant quaternary benzo[c]phenanthridine alkaloid sanguilutine (SL) is a strong inducer of caspase-independent non-apoptotic death in human melanoma cells. Necrostatin-1, a specific inhibitor of necroptosis, completely reversed the cytotoxic effect of SL, suggesting that necroptosis was a predominant type of cell death induced by SL in these cells. In addition, we showed that SL can trigger an autophagic response, as confirmed by GFP-LC3 puncta formation and LC3-II accumulation. Interestingly, we observed a significant decrease in the viability of melanoma cells treated with combination of autophagy inhibitors (3-methyladenine, bafilomycin-A1 and LY294002) and SL. Our results further indicated that autophagy may serve as a pro-survivalmechanism, delaying the induction of necroptosis in melanoma cells.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biological Chemistry

ISSN

1431-6730

e-ISSN

—

Volume of the periodical

393

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

647-658

UT code for WoS article

000307087600011

EID of the result in the Scopus database

—