Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00065980" target="_blank" >RIV/00216224:14110/13:00065980 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/13:00060810

Result on the web

<a href="http://dx.doi.org/10.5966/sctm.2012-0081" target="_blank" >http://dx.doi.org/10.5966/sctm.2012-0081</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5966/sctm.2012-0081" target="_blank" >10.5966/sctm.2012-0081</a>

Result language

angličtina

Original language name

Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Original language description

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high andgradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as asingle-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system ishighly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

STEM CELLS Translational Medicine

ISSN

2157-6564

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

246-254

UT code for WoS article

000317551800002

EID of the result in the Scopus database

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