Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00074774" target="_blank" >RIV/00216224:14110/14:00074774 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/14:PU108618 RIV/62156489:43210/14:43908675

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201300190" target="_blank" >http://dx.doi.org/10.1002/elps.201300190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201300190" target="_blank" >10.1002/elps.201300190</a>

Result language

angličtina

Original language name

Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics

Original language description

Determination of serum mRNA gained a lot of attention in recent years, particularly from the perspective of disease markers. Streptavidin-modified paramagnetic particles (SMPs) seem an interesting technique, mainly due to possible automated isolation andhigh efficiency. The aim of this study was to optimize serum isolation protocol to reduce the consumption of chemicals and sample volume. The following factors were optimized: amounts of (i) paramagnetic particles, (ii) oligo(dT)20 probe, (iii) serum, and (iv) the binding sequence (SMPs, oligo(dT)20, serum vs. oligo(dT)20, serum and SMPs). RNA content was measured, and the expression of metallothionein-2A as possible prostate cancer marker was analyzed to demonstrate measurable RNA content with abilityfor RT-PCR detection.This optimized protocol was also modified to fit needs of automated one-step singletube analysis with identical efficiency compared to conventional setup.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Volume of the periodical

35

Issue of the periodical within the volume

2-3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

306-315

UT code for WoS article

000331899400010

EID of the result in the Scopus database

—