Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00051184" target="_blank" >RIV/00216224:14310/09:00051184 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements
Original language description
The main aim of this work was to study the dependence of oligo adenine isolation efficiency on the chain length by using paramagnetic particles covered by homo eoxythymidines ((dT)25) with subsequent detection by adsorptive transfer technique coupled with square wave voltammetry. For this purpose, the oligonucleotides of the length A5, A10, A15, A20, A25, A30, A35, A40 and poly(A) in various concentrations were chosen. We determined that the isolation efficiency defined as "isolated oligonucleotide concentration''/"given oligonucleotide concentration" was about 55% on average. Sequence A25 demonstrated the best binding onto microparticles surface. (c) 2009 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN
0304-8853
e-ISSN
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Volume of the periodical
321
Issue of the periodical within the volume
10
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
1474-1477
UT code for WoS article
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EID of the result in the Scopus database
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