Single--strand conformation polymorphism of oligonucleotides in their isolation by means of magnetic microparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184685" target="_blank" >RIV/62156489:43210/10:00184685 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Single--strand conformation polymorphism of oligonucleotides in their isolation by means of magnetic microparticles

Original language description

Studies of biomolecules depend strongly on efficiency of their isolation from biological materials. For these purposes the application of paramagnetic particles (MPs) represents a new method. This method, faster and less laborious than commonly used phenol chloroform extraction, is based on the hybridization of target molecules on a surface of magnetic particles. For the isolation of nucleic acids MPs are modified by certain sequences of thymine or other nucleotides. Using MPs we studied the isolation of ODNs (a) with different sequences of adenine and cytosine and (b)with different chain lengths. It was found that single--strand conformation polymorphism (SSCP) of ODNs is crucial phenomena for their isolation.Keywords: paramagnetic particles, oligonucleotides, electrochemical detection

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biochemical Technology

ISSN

0974-2328

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

5

Country of publishing house

IN - INDIA

Number of pages

2

Pages from-to

89-90

UT code for WoS article

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EID of the result in the Scopus database

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