SINGLESTRAND CONFORMATION POLYMORPHISM OF OLIGONUCLEOTIDES IN THEIR ISOLATION BY MEANS OF MAGNETIC MICROPARTICLES
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00051404" target="_blank" >RIV/00216224:14310/10:00051404 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/10:00166809
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
SINGLESTRAND CONFORMATION POLYMORPHISM OF OLIGONUCLEOTIDES IN THEIR ISOLATION BY MEANS OF MAGNETIC MICROPARTICLES
Original language description
Studies of biomolecules depend strongly on efficiency of their isolation from biological materials. Using paramagnetic particles (MPs) represent new method for these purposes. This method, faster and less laborious than commonly used phenol chloroform extraction, is based on hybridization of target molecules on a surface of particles. For the isolation of nucleic acids MPs are modified by certain sequences of thymine or other nucleotides. Using MPs we studied the isolation of ODNs with different sequences of adenine and cytosine and ODNs with different chain lengths. It was found that singlestrand conformation polymorphism (SSCP) of ODNs is crucial phenomena for their isolation.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
ISBN
978-80-7375-396-2
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
233-236
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Jun 23, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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