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MicroRNA profiling of activated and tolerogenic human dendritic cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00077328" target="_blank" >RIV/00216224:14110/14:00077328 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1155/2014/259689" target="_blank" >http://dx.doi.org/10.1155/2014/259689</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1155/2014/259689" target="_blank" >10.1155/2014/259689</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    MicroRNA profiling of activated and tolerogenic human dendritic cells

  • Original language description

    Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-gamma(aDC) or IL-10 and TGF-beta(tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EC - Immunology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Mediators of Inflammation

  • ISSN

    0962-9351

  • e-ISSN

  • Volume of the periodical

    2014

  • Issue of the periodical within the volume

    259689

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    1-10

  • UT code for WoS article

    000334283100001

  • EID of the result in the Scopus database