Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084424" target="_blank" >RIV/00216224:14110/15:00084424 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/15:43907641 RIV/00216305:26620/15:PU113454

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0121674" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0121674</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0121674" target="_blank" >10.1371/journal.pone.0121674</a>

Result language

angličtina

Original language name

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Original language description

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell deathtype due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupledwith fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI-phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progressi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plos One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

"e0121674"

UT code for WoS article

000353889600171

EID of the result in the Scopus database

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