Quantitative phase dynamics of cancer cell populations affected by blue light
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917764" target="_blank" >RIV/62156489:43210/20:43917764 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/20:00114154 RIV/00216305:26220/20:PU136375 RIV/00216208:11110/20:10412112
Result on the web
<a href="https://doi.org/10.3390/app10072597" target="_blank" >https://doi.org/10.3390/app10072597</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/app10072597" target="_blank" >10.3390/app10072597</a>
Alternative languages
Result language
angličtina
Original language name
Quantitative phase dynamics of cancer cell populations affected by blue light
Original language description
Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm2, 208 mJ/cm2 and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm2 x 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm2 x 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Sciences
ISSN
2076-3417
e-ISSN
—
Volume of the periodical
10
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
2597
UT code for WoS article
000533356200396
EID of the result in the Scopus database
2-s2.0-85083427821