The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084871" target="_blank" >RIV/00216224:14110/15:00084871 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1242/jcs.166496" target="_blank" >http://dx.doi.org/10.1242/jcs.166496</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jcs.166496" target="_blank" >10.1242/jcs.166496</a>

Result language
angličtina
Original language name
The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis
Original language description
Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centriolesin response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitylation of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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