DHFR-mediated effects of methotrexate in medulloblastoma and osteosarcoma cells: The same outcome of treatment with different doses in sensitive cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00087437" target="_blank" >RIV/00216224:14110/15:00087437 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/15:00063092 RIV/00209805:_____/15:N0000002
Result on the web
<a href="http://www.spandidos-publications.com/or/33/5/2169" target="_blank" >http://www.spandidos-publications.com/or/33/5/2169</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/or.2015.3819" target="_blank" >10.3892/or.2015.3819</a>
Alternative languages
Result language
angličtina
Original language name
DHFR-mediated effects of methotrexate in medulloblastoma and osteosarcoma cells: The same outcome of treatment with different doses in sensitive cell lines
Original language description
Although methotrexate (MTX) is the most well-known antifolate included in many standard therapeutic regimens, substantial toxicity limits its wider use, particularly in pediatric oncology. Our study focused on a detailed analysis of MTX effects in cell lines derived from two types of pediatric solid tumors: medulloblastoma and osteosarcoma. The main aim of this study was to analyze the effects of treatment with MTX at concentrations comparable to MTX plasma levels in patients treated with high-dose or low-dose MTX. The results showed that treatment with MTX significantly decreased proliferation activity, inhibited the cell cycle at S-phase and induced apoptosis in Daoy and Saos-2 reference cell lines, which were found to be MTX-sensitive. Furthermore, no difference in these effects was observed following treatment with various doses of MTX ranging from 1 to 40 µM.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14327" target="_blank" >NT14327: DHFR- and non-DHFR-mediated effects of methotrexate on cellular level: a study on cell lines derived from pediatric solid tumors</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncology Reports
ISSN
1021-335X
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
5
Country of publishing house
GR - GREECE
Number of pages
7
Pages from-to
2169-2175
UT code for WoS article
000353180900010
EID of the result in the Scopus database
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