Electrochemical chip-based genomagnetic assay for detection of high-risk human papillomavirus DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00108299" target="_blank" >RIV/00216224:14110/16:00108299 - isvavai.cz</a>

Alternative codes found

RIV/00209805:_____/16:N0000026 RIV/65269705:_____/16:00066001

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0956566316303098?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0956566316303098?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bios.2016.04.035" target="_blank" >10.1016/j.bios.2016.04.035</a>

Result language

angličtina

Original language name

Electrochemical chip-based genomagnetic assay for detection of high-risk human papillomavirus DNA

Original language description

Cervical cancer, being the fourth leading cause of cancer death in women worldwide, predominantly originates from a persistent infection with a high-risk human papillomavirus (HPV). Detection of DNA sequences from these high-risk strains, mostly HPV-16 and HPV-18, represents promising strategy for early screening, which would help to identify women with higher risk of cervical cancer. In developing countries, inadequate screening options lead to disproportionately high mortality rates, making a fast and inexpensive detection schemes highly important. Electrochemical sensors and assays offer an alternative to current methods of detection. We developed an electrochemical-chip based assay, in which target HPV DNA is captured via magnetic bead-modified DNA probes, followed by an antidigoxigenin-peroxidase detection system at screen-printed carbon electrode chips, enabling parallel measurements of eight samples simultaneously. We show sensitive detection in attomoles of HPV DNA, selective discrimination between HPV-16 and HPV-18 and good reproducibility. Most importantly, we show application of the assay into both cancer cell lines and cervical smears from patients. The electrochemical results correlated well with standard methods, making this assay potentially applicable in clinical practice. (C) 2016 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOSENSORS &amp; BIOELECTRONICS

ISSN

0956-5663

e-ISSN

—

Volume of the periodical

83

Issue of the periodical within the volume

SEP 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

300-305

UT code for WoS article

000376802300042

EID of the result in the Scopus database

2-s2.0-84964425483