NANOSTRUCTURE AND BIOACTIVITY OF MOUSE LUNG EXTRACELLULAR MATRIX SCAFFOLDS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00100758" target="_blank" >RIV/00216224:14110/18:00100758 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/18:00070389
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
NANOSTRUCTURE AND BIOACTIVITY OF MOUSE LUNG EXTRACELLULAR MATRIX SCAFFOLDS
Original language description
Extracellular matrix (ECM) forms an essential part of tissue microenvironment. Compositional and structural properties of ECM modulate behaviour of cells, including their differentiation, proliferation, and turnover. Here we aimed at detailed characterization of the nanostructure of lung ECM and evaluation of its bioactivity on cells grown in vitro. Mouse lungs were decellularized using 0.2 % sodium dodecyl sulphate, hypotonic solutions, and DNase. Morphological analysis of the resulting ECM scaffolds was performed by means of transmission and scanning electron microscopy. The ECM scaffolds retained 3D architecture of the main lung anatomical regions: the alveolar region and the blood/ airway network. The region-specific ECM nanotopology and organization of ECM macromolecules such as fibres of collagen, elastin, and fibrillin were characterized. The lung ECM scaffolds were also homogenized and applied as a supplement to growth medium on primary lung cells in vitro to test the bioactivity of lung ECM. We demonstrate that homogenized ECM does not have a negative impact on the proliferation rate of primary lung cells. In conclusion, we herein show at nanoscale the morphological characteristics of lung ECM and demonstrate that lung ECM produced by decellularization procedure is compatible with in vitro cultured lung cells. These findings add to a better understanding of cells with their natural environment and can be valuable when designing applications of ECM for purposes in tissue engineering.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017)
ISBN
9788087294819
ISSN
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e-ISSN
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Number of pages
7
Pages from-to
560-566
Publisher name
TANGER LTD
Place of publication
SLEZSKA
Event location
Brno, CZECH REPUBLIC
Event date
Oct 18, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000452823300092