Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00136048" target="_blank" >RIV/00216224:14110/24:00136048 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3674-9_3" target="_blank" >10.1007/978-1-0716-3674-9_3</a>
Alternative languages
Result language
angličtina
Original language name
Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
Original language description
Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/NV16-31501A" target="_blank" >NV16-31501A: Engineering of epithelia: Cells and protocols for regenerative medicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
3D Cell Culture. Methods in Molecular Biology
ISBN
9781071636732
Number of pages of the result
13
Pages from-to
21-33
Number of pages of the book
336
Publisher name
Humana
Place of publication
New York
UT code for WoS chapter
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