Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00104139" target="_blank" >RIV/00216224:14110/18:00104139 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s12885-018-4508-1" target="_blank" >http://dx.doi.org/10.1186/s12885-018-4508-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12885-018-4508-1" target="_blank" >10.1186/s12885-018-4508-1</a>

Result language

angličtina

Original language name

Endogenous H2S producing enzymes are involved in apoptosis induction in clear cell renal cell carcinoma

Original language description

Background: Knowledge about the expression and thus a role of enzymes that produce endogenous H2S-cystathionine-beta-synthase, cystathionine gamma-lyase and mercaptopyruvate sulfurtransferase - in renal tumors is still controversial. In this study we aimed to determine the expression of these enzymes relatively to the expression in unaffected part of kidney from the same patient and to found relation of these changes to apoptosis. To evaluate patient's samples, microarray and immunohistochemistry was used. Methods: To determine the physiological importance, we used RCC4 stable cell line derived from clear cell renal cell carcinoma, where apoptosis induction by a mixture of five chemotherapeutics with/without silencing of H2S-producing enzymes was detected. Immunofluorescence was used to determine each enzyme in the cells. Results: In clear cell renal cell carcinomas, expression of H2S-producing enzymes was mostly decreased compared to a part of kidney that was distal from the tumor. To evaluate a potential role of H2S-producing enzymes in the apoptosis induction, we used RCC4 stable cell line. We have found that silencing of cystathionine-beta-synthase and cystathionine.-lyase prevented induction of apoptosis. Immunofluorescence staining clearly showed that these enzymes were upregulated during apoptosis in RCC4 cells. Conclusion: Based on these results we concluded that in clear cell renal cell carcinoma, reduced expression of the H2S-producing enzymes, mainly cystathionine gamma-lyase, might contribute to a resistance to the induction of apoptosis. Increased production of the endogenous H2S, or donation from the external sources might be of a therapeutic importance in these tumors.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30105 - Physiology (including cytology)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Cancer

ISSN

1471-2407

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

591

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1-8

UT code for WoS article

000433334000005

EID of the result in the Scopus database

2-s2.0-85047467781