A new simple approach to estimation of membrane capacitance from current responses to voltage clamp steps

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00118630" target="_blank" >RIV/00216224:14110/20:00118630 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0079610720300341?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0079610720300341?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pbiomolbio.2020.04.005" target="_blank" >10.1016/j.pbiomolbio.2020.04.005</a>

Result language

angličtina

Original language name

A new simple approach to estimation of membrane capacitance from current responses to voltage clamp steps

Original language description

A variety of techniques of cell capacitance measurement have been proposed and applied in cellular electrophysiology. They are mostly based on the evaluation of membrane current responses to small changes in the membrane voltage. One of the currently used approaches applies the least-squares fit of an exponential current decay in response to voltage clamped rectangular pulses. In this study, we propose an alternative simpler approach to evaluation of the exponential parts in the current responses to square wave stimulation and present preliminary results of membrane capacitance evaluation. It is based on the property of the exponential function that has not yet been used to measure membrane capacitance. The time constant and the asymptote of the exponential waveform are unambiguously determined by the values read at three points separated by a constant time interval. In order to minimize the effect of noise and deviations from the exponential waveform, the triplet of points is designed to slide along the time axis. The results of the proposed approach and those previously evaluated by the least squares method are comparable. The method described may be advantageous for continuously recording changes in membrane capacitance. (C) 2020 Elsevier Ltd. All rights reserved.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NV16-30571A" target="_blank" >NV16-30571A: Clinical significance and electrophysiological evaluation of KCNQ1 gene mutation c.926C>T (p.T309I) as a possible long QT syndrome founder mutation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

ISSN

0079-6107

e-ISSN

—

Volume of the periodical

157

Issue of the periodical within the volume

NOV 2020

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

18-23

UT code for WoS article

000582745400004

EID of the result in the Scopus database

2-s2.0-85086374991