Cytoplasmic maturation in human oocytes: an ultrastructural study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00118831" target="_blank" >RIV/00216224:14110/21:00118831 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/biolreprod/article/104/1/106/5909494" target="_blank" >https://academic.oup.com/biolreprod/article/104/1/106/5909494</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biolre/ioaa174" target="_blank" >10.1093/biolre/ioaa174</a>
Alternative languages
Result language
angličtina
Original language name
Cytoplasmic maturation in human oocytes: an ultrastructural study
Original language description
Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here, we report our findings from the ultrastructural analysis of 69 unfertilized human oocytes from 34 young and healthy egg donors. By comparison of samples fixed at three consecutive developmental stages, we explored how ooplasmic architecture changes during meiotic maturation in vitro. The morphometric image analysis supported observation that the major reorganization of cytoplasm occurs before polar body extrusion. The organelles initially concentrated around prophase nucleus were repositioned toward the periphery and evenly distributed throughout the ooplasm. As maturation progressed, distinct secretory apparatus appeared to transform into cortical granules that clustered underneath the oocyte's surface. The most prominent feature was the gradual formation of heterologous complexes composed of variable elements of endoplasmic reticulum and multiple mitochondria with primitive morphology. Based on the generated image dataset, we proposed a morphological map of cytoplasmic maturation, which may serve as a reference for future comparative studies. In conclusion, this work improves our understanding of human oocyte morphology, cytoplasmic maturation, and intracellular factors defining human egg quality. Although this analysis involved spare oocytes completing development in vitro, it provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
<a href="/en/project/GJ19-14990Y" target="_blank" >GJ19-14990Y: Ultrastructural markers of human oocyte quality</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of Reproduction
ISSN
0006-3363
e-ISSN
1529-7268
Volume of the periodical
104
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
106-116
UT code for WoS article
000609888100010
EID of the result in the Scopus database
2-s2.0-85100125565