A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00121913" target="_blank" >RIV/00216224:14110/21:00121913 - isvavai.cz</a>
Result on the web
<a href="https://bio-protocol.org/e3996" target="_blank" >https://bio-protocol.org/e3996</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21769/BioProtoc.3996" target="_blank" >10.21769/BioProtoc.3996</a>
Alternative languages
Result language
angličtina
Original language name
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
Original language description
The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding how dysregulation can lead to breast cancer initiation and progression. Three-dimensional (3D) mammary organoids offer many exciting possibilities for the study of tissue development and breast cancer. In the present protocol derived from Sumbal et al., we describe a straightforward 3D organoid system for the study of lactation and involution ex vivo. We use primary and passaged mouse mammary organoids stimulated with fibroblast growth factor 2 (FGF2) and prolactin to model the three cycles of mouse mammary gland lactation and involution processes. This 3D organoid model represents a valuable tool to study late postnatal mammary gland development and breast cancer, in particular postpartum-associated breast cancer.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bio-protocol
ISSN
2331-8325
e-ISSN
2331-8325
Volume of the periodical
11
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
„e3996“
UT code for WoS article
000642293100012
EID of the result in the Scopus database
2-s2.0-85116293259