Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F22%3A00125709" target="_blank" >RIV/00216224:14110/22:00125709 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/22:N0000098 RIV/65269705:_____/22:00075973

Result on the web

<a href="https://sciendo.com/it/article/10.2478/acb-2022-0001" target="_blank" >https://sciendo.com/it/article/10.2478/acb-2022-0001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2022-0001" target="_blank" >10.2478/acb-2022-0001</a>

Result language

angličtina

Original language name

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Original language description

The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-ra-pid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P &lt; 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.

Czech name

—

Czech description

—

Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

30214 - Obstetrics and gynaecology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

2544-3577

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

6

Pages from-to

1-6

UT code for WoS article

—

EID of the result in the Scopus database

—