Proteome Analysis of Seven Treponema pallidum subsp. pallidum Strains Grown In Vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00142266" target="_blank" >RIV/00216224:14110/25:00142266 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00624" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00624</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jproteome.5c00624" target="_blank" >10.1021/acs.jproteome.5c00624</a>
Alternative languages
Result language
angličtina
Original language name
Proteome Analysis of Seven Treponema pallidum subsp. pallidum Strains Grown In Vitro
Original language description
Treponema pallidum subsp. pallidum (T. pallidum), the fastidious causative agent of syphilis, has become more accessible for research with its recently developed in vitro cultivation method. In this work, the proteomes of seven T. pallidum strains (Nichols-like: DAL-1, Haiti B, and Madras; SS14-like: SS14, Mexico A, Philadelphia 1, and Grady), cultivated in vitro, were analyzed in biological triplicates by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS/MS data were processed against their corresponding genomes using various annotation algorithms (DFAST, PGAP, Prodigal, Prokka, RAST, GeneMarkS, and manual GenBank annotation). Additionally, ORFfinder was used to predict all ORFs encoding polypeptides exceeding 50 amino acids. While the RAST algorithm predicted the highest number of genes per genome, GeneMarkS offered the best coverage of annotated genes (up to 88.9%). By combining annotations from seven T. pallidum strains, we identified 911 unique treponemal proteins (74.9% of 1216 predicted sequences). The confidence of protein identifications was high, with 85.5% identified by two or more peptides and 72.4% by three or more peptides. Overall, 51 proteins showed statistically significant quantitative differences in intensity across T. pallidum strains. Furthermore, our proteome analysis revealed detectable quantitative proteomic differences between strains in the Nichols-like and SS14-like groups.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Proteome Research
ISSN
1535-3893
e-ISSN
1535-3907
Volume of the periodical
24
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
6091-6100
UT code for WoS article
001591527500001
EID of the result in the Scopus database
2-s2.0-105024020370