Wnt1's Differential Effects on Craniofacial Bone and Tooth Development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00144367" target="_blank" >RIV/00216224:14110/25:00144367 - isvavai.cz</a>
Result on the web
<a href="https://journals.sagepub.com/doi/10.1177/00220345251336191" target="_blank" >https://journals.sagepub.com/doi/10.1177/00220345251336191</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/00220345251336191" target="_blank" >10.1177/00220345251336191</a>
Alternative languages
Result language
angličtina
Original language name
Wnt1's Differential Effects on Craniofacial Bone and Tooth Development
Original language description
The development of craniofacial bones and teeth relies heavily on the Wnt signaling pathway, yet the specific mechanisms and Wnt variants involved remain under continual investigation. Using publicly available single-cell sequencing data from the mouse incisor, we reveal Wnt1 expression across dental structures and investigate its role using a Col1a1-dependent Wnt1 transgenic mouse model. Inducing Wnt1 early on affects craniofacial bone without disturbing tooth development, but prolonged embryonic induction leads to postnatal mortality with osteopetrosis-like bone overgrowth and malformed teeth. While tooth formation was initially unaffected by postnatal Wnt1 induction, prolonged activation impaired tooth root formation and odontoblast differentiation, resulting in shortened roots and thinner dentin. Three-dimensional micro-computed tomography quantification reveal that both embryonic and postnatal activation of Wnt1 significantly increase neural crest-derived craniofacial bone volume, whereas mesenchymal-derived craniofacial bones are unaffected. Importantly, osteoclastogenesis is suppressed by Wnt1 in a dose-dependent manner, revealed through bulk RNA sequencing and in vitro experiments. These findings emphasize the differential effects of Wnt1 on bone development based on origin and highlight its role in modulating osteoclast activity, indicating broader implications for craniofacial development and potential therapeutic avenues.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30208 - Dentistry, oral surgery and medicine
Result continuities
Project
<a href="/en/project/GA23-06160S" target="_blank" >GA23-06160S: Fluctuation of the stem cell niche as a source of tissue adaptability in health and disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Dental Research
ISSN
0022-0345
e-ISSN
1544-0591
Volume of the periodical
104
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1415-1424
UT code for WoS article
001500237400001
EID of the result in the Scopus database
2-s2.0-105008065551