SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F24%3A00139148" target="_blank" >RIV/00216224:14160/24:00139148 - isvavai.cz</a>
Result on the web
<a href="https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB" target="_blank" >https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
Original language description
Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-00236S" target="_blank" >GA22-00236S: Development of novel fluorescent labeling tags for electrophoretic analysis of glycoproteins associated with breast cancer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů