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Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002294" target="_blank" >RIV/00216224:14310/00:00002294 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

  • Original language description

    On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in theirrestriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective,

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2000

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    9th International Symposium on Staphylococci and Staphylococcal Infections

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

  • Publisher name

    JDS

  • Place of publication

    Kolding

  • Event location

  • Event date

  • Type of event by nationality

  • UT code for WoS article