Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006633" target="_blank" >RIV/00216224:14310/02:00006633 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Multiplex PCR for detection of <I>Staphylococcus aureus</I> prophages
Original language description
Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.Thedesigned primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA301%2F02%2F1505" target="_blank" >GA301/02/1505: Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
10th International Symposium on Staphylococci and Staphylococcal Infections
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
66
Publisher name
Juntendo University
Place of publication
Tsukuba, Japan
Event location
October 16-19, 2002
Event date
Jan 1, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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